Scientist I

Job Title: Scientist I
Job Location: San Diego, CA 92121
Job Duration: 6 Months 
Dept: Genetic Medicine Technology (gRED)

Description

Position Summary:

The Scientist I will be a vital contributor to our non-viral cell and gene therapy delivery platform, primarily focused on R&D for innovative nucleic acid payload technologies. This role involves evaluating novel mRNA and DNA designs using a range of molecular biology techniques, including plasmid cloning, in vitro validation, and the production of in vitro-transcribed mRNA. To verify successful expression, the scientist will utilize assays such as Western blot, qRT-PCR, and luciferase reporter assays.

Responsibilities:

• Design and clone expression vectors and IVT donor plasmids to support research initiatives.

• Execute DNA and mRNA transfections in mouse and human cell lines.

• Assess nucleic acid expression through molecular methods like qPCR, reporter assays and Western blotting.

• Maintenance of detailed electronic laboratory notebooks (ELN) and preparation of summary slides

 

Requirements, Knowledge, Skills, and Abilities:

• Education/Experience: Bachelor's degree with 5+ years or a Master's degree with 2+ years of experience in molecular biology, pharmaceutical sciences, or a related field.

• Molecular Biology: Required proficiency in advanced cloning (Gibson assembly, Golden Gate, site-directed mutagenesis) and IVT.

• Functional Assays: Hands-on expertise in measuring transgene delivery success via reporter assays and qRT-PCR.

• Cell Culture: Preferred experience in genetic manipulation of cell lines and primary cells (e.g., T cells, monocytes, hepatocytes).

• Software/Tools: Desired proficiency in Snapgene, Prism, and MS Office.

• Communication: Exceptional oral and written skills with a meticulous attention to detail.

 

At SPECTRAFORCE, we are committed to maintaining a workplace that ensures fair compensation and wage transparency in adherence with all applicable state and local laws. This position's starting pay is: $44.00/hr.